Chaos ensues after Princess Peach bans anime following the Anime Challenge incident, while Meggy and the Inklings have been kidnapped by an Anime Cartel. With things going out of control, it's up to Mario, SMG4, and their friends to work together to lift the ban, prompt a rescue mission to Anime Island, and put an end to the crisis that has been masterminded by the cartel's mysterious leader behind the scenes.

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